Fields of Research
Intracellular signaling
Molecular basis of disease
Protein structure and function
Research Summary
I am researching the binding affinity of different AKAPs to the two classes of PKA. I use a microscopy technique called TIRF (total internal reflection fluorescence) to image at the cell membrane, and perform FRAP (fluorescence recovery after photobleaching) to measure protein mobility. This in situ technique allows me to ask two questions about AKAP-PKA binding in living cells: Do so called “selective” AKAPs anchor both subtypes of PKA? How does the composition of AKAP signaling islands change during disease?
Research Statement
A-kinase anchoring proteins (AKAPs) regulate signaling events by constraining kinases, phosphatases, and their substrates at specific locations in the cell. I am interested in the physical features of AKAPs that facilitate specificity of protein kinase A anchoring, and how AKAP signaling islands behave in disease states.
Awards and Honors
Faculty
- Building:
- HSB
- Room:
- K-326
- Box:
- 357750
- Phone:
- 206-221-0517
- Web Link:
- https://www.ncbi.nlm.nih.gov/myncbi/16GsfyRmDumEwh/bibliography/public/
Lab
- Building:
- HSB
- Room:
- K-326
- Box:
- 357750
- Phone:
- 206-221-0517
- Web Link:
- https://www.ncbi.nlm.nih.gov/myncbi/16GsfyRmDumEwh/bibliography/public/
Publications
Gopalan J, Omar MH, Roy A, Cruz NM, Falcone J, Jones KN, Forbush KA, Himmelfarb J, Freedman BS, Scott JD. Targeting an anchored phosphatase-deacetylase unit restores renal ciliary homeostasis. Elife. 2021 Jul 12;10:e67828. doi: 10.7554/eLife.67828. PMID: 34250905; PMCID: PMC8291974.
Bogdan D*, Falcone J*, Kanjiya MP, Park SH, Carbonetti G, Studholme K, Gomez M, Lu Y, Elmes MW, Smietalo N, Yan S, Ojima I, Puopolo M, Kaczocha M. Fatty acid-binding protein 5 controls microsomal prostaglandin E synthase 1 (mPGES-1) induction during inflammation. J Biol Chem. 2018 Apr 6;293(14):5295-5306. doi: 10.1074/jbc.RA118.001593. Epub 2018 Feb 13. PubMed PMID: 29440395; PubMed Central PMCID: PMC5892576.
Yan S, Elmes MW, Tong S, Hu K, Awwa M, Teng GYH, Jing Y, Freitag M, Gan Q, Clement T, Wei L, Sweeney JM, Joseph OM, Che J, Carbonetti GS, Wang L, Bogdan DM, Falcone J, Smietalo N, Zhou Y, Ralph B, Hsu HC, Li H, Rizzo RC, Deutsch DG, Kaczocha M, Ojima I. SAR studies on truxillic acid mono esters as a new class of antinociceptive agents targeting fatty acid binding proteins. Eur J Med Chem. 2018 Jun 25;154:233-252. doi: 10.1016/j.ejmech.2018.04.050. Epub 2018 May 26. PubMed PMID: 29803996; PubMed Central PMCID: PMC5999033.